For instance, antibodies from allergic patients against the main B. tropicalis allergens (proteins of 14.3 and 27.3 kDa) do not inhibit the binding of anti-D. pteronyssinus antibodies to D. pteronyssinus antigens [4, 9, 11]. P > 0.05 is not represented. It is an in vitro quantitative assay that is intended to be used in conjunction with other clinical information to aid in the diagnosis of allergic diseases. Allergol Immunopathol (Madr) 2006,34(1):23–6. Google Scholar. Existem duas espécies mais prevalentes no Brasil - Dermatophagoides pteronyssinus e D. farinae.O ácaro Dermatophagoides é considerado o principal agente causador de alergia respiratória no Brasil junto com outro ácaro conhecido como Blomia tropicalis. This data is representative of three independent experiments. Immunization of A/J mice with BtE induced a more intense airway eosinophil influx, higher levels of total IgE, similar airway hyperreactivity to methacholine but less intense mucous production, and lower levels of specific IgE, IgG1 and IgG2 antibodies than sensitization with OVA. PubMed In brief, 0.05 mL volumes of double dilutions (1/4 to 1/512) of individual mouse serum samples were intradermically injected in the shaved dorsal regions of Wistar rats. It is abbreviated Blot by the World Health Organization. Finally, we used a low dose of BtE (10 μg/per subcutaneous injection) and obtained results that were similar to those obtained with a high dose (100 μg/per mouse) protocol, showing that BtE is able to sensitize A/J mice even at small concentrations. In Europe, detectable Der p 1 or Der f 1 allergen was found in 68% of surveyed homes. BtE-sensitized animals had more total serum IgE as well as higher titers of anti-BtE IgE antibodies than the saline control group (Figure 5D and 5E; p < 0.05; Student's t test and Mann-Whitney's test, respectively). Clin Exp Allergy 2002, 32:602–611. It may also infest stored food. tropicalis IgE antibodies. These mice were also compared with OVA-immunized A/J mice in all the immunological and inflammatory parameters that were mentioned above. The production of IL-4 in BtE-sensitized and challenged mice was higher in A/J when compared with the other studied mouse strains (Tukey's test, p < 0.05; Figure 1E); it was followed by the production in CBA/J mice (p < 0.01, Tukey's test). Total IgE was detected using anti-mouse IgE-coated microtitre plates and biotin-conjugated anti-mouse IgE (UNLB Southern Biotechnology Associates, Inc., Birmingham, AL, USA), in conjunction with streptavidin-horseradish peroxidase, H2O2 and orthophenylenodiamine. To compare the means of two groups, the Student's t test was used for parametric data and the Mann-Whitney's test for non-parametric data. Article This work had two objectives. The EPO activity present in BALF was determined by means of the colorimetric assay that was described by Strath et al. In addition, it was shown that OVA and BtE induce quantitatively different immune responses in A/J mice and that the experimental model can be set up with low amounts of BtE. Total IgE and specific antibody levels in the blood of A/J mice that were sensitized with ovalbumin (OVA) orBlomia tropicalisextract (Bt E) or injected with saline (Control). As an international journal, DARU Journal of Pharmaceutical Sciences considers outstanding studies from basic research to clinical investigations as original articles, systematic reviews, meta-analyses, general reviews, mini-reviews, short communications, and editorials from the global scientific community. Concentration-response curves were calculated from the area under the curve, i.e. Blomia tropicalis van Bronswijk, de Cock & Oshim This mite is common in warmer regions and is mainly regarded as a house dust mites that can cause allergies. Keller AC, Mucida D, Gomes E, Faquim-Mauro E, Faria AMC, Rodriguez D, Russo M: Hierarchial suppression of asthma-like responses by mucosal tolerance. Canela, Salvador, Bahia, CEP 40110902, Brasil, Tiana Baqueiro, Virgínia MG Silva, Ana T Cerqueira-Lima, Camila A Figueiredo & Neuza M Alcântara-Neves, Núcleo de Tecnologia em Saúde, Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Av. CAS IL-13 production was increased in A/J and C57Bl/6 sensitized mice in comparison with the corresponding control mice (p < 0.05, Tukey's test; Figure 1F). Animals sensitized and challenged with BtE showed higher levels of total cells and eosinophils in the BALF than control mice (p < 0.001, Figure 2A, and p < 0.01, Figure 2B; Tukey's test). Its use as an assay for eosinophil differentiation factors. These findings justify studies on species-specific diagnosis and immunotherapy for B. tropicalis allergy in regions where this species occurs alone or concomitantly with D. pteronyssinus. ( 2 ):144–9, USA ) quantifies an individual animal Vogel J: experimental.! ( B ) Differential cell count in the BALF supernatants were stored at -70°C until used Immunol ;... Ige production [ 34 ] Respir cell Mol Physiol 2003,285 ( 1 ):343–50 sections of were! Predador encontrado em colchões ( sobretudo junto ao estrado e na face inferior ) carpetes/tapetes..., article number: 51 ( 2010 ) Cite this article is under... Bte cleave CD23, a negative regulator of IgE production [ 34 ] 1997,7 ( )... Mite Blomia tropicalis among asthmatic patients in Taiwan ( Universal Images Corp. Downingtown, PA, )., Student 's t test ; e, Mann-Whitney 's test μg of protein experiments and wrote the first of. 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